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fos si fos  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology fos si fos
    Fos Si Fos, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c+fos+sirna/c-Fos+siRNA/pm39998071-345-16-29
    Average 93 stars, based on 59 article reviews
    fos si fos - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    shRNA:

    Article Title: O-GlcNAc regulates MTA1 transcriptional activity during breast cancer cells genotoxic adaptation
    Article Snippet: .. OGT siRNA (#sc-40781), c-Fos siRNA (#sc-29221) and MTA1 shRNA (#sc-35981-SH) were purchased from Santa Cruz Biotechnology. ..

    Article Title: O-GlcNAc modification regulates MTA1 transcriptional activity during breast cancer cell genotoxic adaptation.
    Article Snippet: Background: Chromatin modifier metastasis-associated protein 1 (MTA1), closely associated with tumor angiogenesis in breast cancer, plays an important role in gene expression and cancer cell behavior.. Recently, an association between O-GlcNAc transferase (OGT) and MTA1 was identified by mass spectroscopy.. However, the potential relationship between MTA1 and O-GlcNAc modification has not yet explored.

    Negative Control:

    Article Title: Nardilysin-regulated scission mechanism activates polo-like kinase 3 to suppress the development of pancreatic cancer
    Article Snippet: .. C-Fos siRNA and scramble RNA (negative control) were purchased from Santa Cruz Biotechnology (SC-29221), and 20 nM siRNA was transfected into HPNE cells with Lipofectamine RNAiMax reagent (Life Technologies). .. ON-TARGETplus SMARTpool human Plk3 siRNA (L-003257-00-0005) and ON-TARGETINGplus Non-targeting pool (D-001810-10-05) were obtained from Dharmacon.

    Article Title: Beta-adrenoceptor Activation by Norepinephrine Enhances Lipopolysaccharide-induced Matrix Metalloproteinase-9 Expression Through the ERK/JNK-c-Fos Pathway in Human THP-1 Cells
    Article Snippet: To assess gelatinolytic activity, the gels were incubated for 24 h at 37°C in a developing buffer (LC2671, Life Technology) and stained with Coomassie Brilliant Blue solution. .. c-Fos siRNA (catalog number sc-29221) and negative control siRNA (catalog number sc-37007) were purchased from Santa Cruz Biotechnology, Inc. For the transfection procedure, cells were grown to 70%–80% confluence, and c-Fos siRNA and control siRNA were transfected using LipofetamineTM2000 (Invitrogen, Carlsbad, CA) according to the manufacturer's instructions. ..

    Article Title: Nardilysin-Regulated Scission Mechanism Activates Polo-like Kinase 3 to Suppress the Development of Pancreatic Cancer
    Article Snippet: .. C-Fos siRNA and scramble RNA (negative control) were purchased from Santa Cruz Biotechnology (SC-29221), and 20 nM siRNA was transfected into HPNE cells with Lipofectamine RNAiMax reagent (Life Technologies). .. ON-TARGETplus SMARTpool human Plk3 siRNA (L-003257-00-0005) and ON-TARGETINGplus Non-targeting pool (D-001810-10-05) were obtained from Dharmacon.

    Transfection:

    Article Title: Nardilysin-regulated scission mechanism activates polo-like kinase 3 to suppress the development of pancreatic cancer
    Article Snippet: .. C-Fos siRNA and scramble RNA (negative control) were purchased from Santa Cruz Biotechnology (SC-29221), and 20 nM siRNA was transfected into HPNE cells with Lipofectamine RNAiMax reagent (Life Technologies). .. ON-TARGETplus SMARTpool human Plk3 siRNA (L-003257-00-0005) and ON-TARGETINGplus Non-targeting pool (D-001810-10-05) were obtained from Dharmacon.

    Article Title: Beta-adrenoceptor Activation by Norepinephrine Enhances Lipopolysaccharide-induced Matrix Metalloproteinase-9 Expression Through the ERK/JNK-c-Fos Pathway in Human THP-1 Cells
    Article Snippet: To assess gelatinolytic activity, the gels were incubated for 24 h at 37°C in a developing buffer (LC2671, Life Technology) and stained with Coomassie Brilliant Blue solution. .. c-Fos siRNA (catalog number sc-29221) and negative control siRNA (catalog number sc-37007) were purchased from Santa Cruz Biotechnology, Inc. For the transfection procedure, cells were grown to 70%–80% confluence, and c-Fos siRNA and control siRNA were transfected using LipofetamineTM2000 (Invitrogen, Carlsbad, CA) according to the manufacturer's instructions. ..

    Article Title: Nardilysin-Regulated Scission Mechanism Activates Polo-like Kinase 3 to Suppress the Development of Pancreatic Cancer
    Article Snippet: .. C-Fos siRNA and scramble RNA (negative control) were purchased from Santa Cruz Biotechnology (SC-29221), and 20 nM siRNA was transfected into HPNE cells with Lipofectamine RNAiMax reagent (Life Technologies). .. ON-TARGETplus SMARTpool human Plk3 siRNA (L-003257-00-0005) and ON-TARGETINGplus Non-targeting pool (D-001810-10-05) were obtained from Dharmacon.

    Article Title: c-Fos mediates α1, 2-fucosyltransferase 1 and Lewis y expression in response to TGF-β1 in ovarian cancer.
    Article Snippet: .. When the cells reached 60% confluence, scramble siRNA, c-Jun siRNA and c-Fos siRNA obtained from Santa Cruz Biotechnology, Inc. were transfected into the cell line, respectively, using Lipofectamine RNA iMAX transfection reagent (Invitrogen). ..

    Control:

    Article Title: MicroRNA-101 downregulation increases C-Fos expression and contributes to the pathogenesis of non-small cell lung cancer
    Article Snippet: MiRNA mimics, C-Fos siRNA and transfection The human miR-101 duplex mimics (miR-101) and negative control oligonucleotide duplex mimics (miR-NC) were designed and obtained from Ribobio (Guangzhou, China). .. C-Fos siRNA and control siRNA oligonucleotides were purchased from Santa Cruz (Santa Cruz, USA). .. MiRNA or siRNA transfection was performed using Lipofectamine 2000 (Invitrogen, USA) following the manufacturer’s protocol.

    Article Title: Beta-adrenoceptor Activation by Norepinephrine Enhances Lipopolysaccharide-induced Matrix Metalloproteinase-9 Expression Through the ERK/JNK-c-Fos Pathway in Human THP-1 Cells
    Article Snippet: To assess gelatinolytic activity, the gels were incubated for 24 h at 37°C in a developing buffer (LC2671, Life Technology) and stained with Coomassie Brilliant Blue solution. .. c-Fos siRNA (catalog number sc-29221) and negative control siRNA (catalog number sc-37007) were purchased from Santa Cruz Biotechnology, Inc. For the transfection procedure, cells were grown to 70%–80% confluence, and c-Fos siRNA and control siRNA were transfected using LipofetamineTM2000 (Invitrogen, Carlsbad, CA) according to the manufacturer's instructions. ..

    Recombinant:

    Article Title: LIF, a Novel Myokine, Protects Against Amyloid-Beta-Induced Neurotoxicity via Akt-Mediated Autophagy Signaling in Hippocampal Cells
    Article Snippet: PD98059 was purchased from Calbiochem. .. Recombinant human LIF was purchased from Peprotech. c-fos siRNA was purchased from Santa Cruz Biotechnology. .. HT-22 mouse hippocampal cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Invitrogen) supplemented with 10% fetal bovine serum and antibiotics at 37°C in a 5% CO 2 atmosphere.



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    a Left, immunoblot of Plk3 expression under a Dox-inducible system in HPNE cells treated with Dox for the indicated times. Middle and right, apoptosis-inducing activity of HPNE/iPlk3 as evaluated using Annexin V/PI staining and flow cytometry analysis. b , c Immunoblots of cleaved PARP in HPDE cells ( b ) and PDAC cells ( c ) grown in suspension (Sus. Culture) on polyHEMA pre-coated plates at the indicated times. C.B.S., Coomassie blue–stained protein bands as a loading control. d , e Immunoblots of cleaved PARP in HPDE cells with stable <t>shRNA-mediated</t> knockdown of Plk3 ( d ) and stably reconstituted with Flag-Plk3 ( e ). f Immunoblot of p72Plk3 and p41Plk3 in PANC-1 cells transfected with N-terminal Flag-p72Plk3. g Immunoblot of p72Plk3 and p41Plk3 in a panel of PDX cell lines grown in suspension culture for 48 h. h – j Immunoblots of p72Plk3 and p41Plk3 in HPDE cells ( h ), PATC50 cells, PANC-1 cells ( i ), and 293T cells ( j ) grown in suspension for the indicated times. k , l Immunoblot of p72Plk3, p41Plk3 expression ( k ) and flow cytometry analysis of apoptosis-inducing activity ( l ) of indicated PDAC cells that were lentivirally transduced to express the sgRNA targeting Plk3 or non-targeting control sgRNA. Cells grow on tissue culture plates (Attached) or polyHEMA-coated plates in suspension (Suspension). The p41Plk3:p72Plk3 ratios are shown at the bottom. m , n Immunoblots of p72Plk3 and p32Plk3 in PANC-1 cells grown in suspension condition. Expression of Plk3 aa 354-646 in lane 4 in ( n ) was used as a positive control. o Immunoblot of p38Plk1 expression in PANC-1 cells transfected with Myc-p68Plk1 in suspension culture for the indicated times. p Immunoblot of Plk1 cleavage in the indicated PDAC cells transfected with Plk1 shRNA. The p41Plk3:p72Plk3 ratios or p32Plk3:p72Plk3 ratios are shown at the bottom in ( f , h – j , and m ). Data are representative of two independent experiments with similar results ( a left, b – k, m – p ). n = 3 independent experiments ( a , l ). Error bars, mean ± SEM ( a right, l ), two-tailed unpaired t -test ( a right), ANOVA analysis (l ). Source data are provided as a Source Data file.
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    Image Search Results


    a Left, immunoblot of Plk3 expression under a Dox-inducible system in HPNE cells treated with Dox for the indicated times. Middle and right, apoptosis-inducing activity of HPNE/iPlk3 as evaluated using Annexin V/PI staining and flow cytometry analysis. b , c Immunoblots of cleaved PARP in HPDE cells ( b ) and PDAC cells ( c ) grown in suspension (Sus. Culture) on polyHEMA pre-coated plates at the indicated times. C.B.S., Coomassie blue–stained protein bands as a loading control. d , e Immunoblots of cleaved PARP in HPDE cells with stable shRNA-mediated knockdown of Plk3 ( d ) and stably reconstituted with Flag-Plk3 ( e ). f Immunoblot of p72Plk3 and p41Plk3 in PANC-1 cells transfected with N-terminal Flag-p72Plk3. g Immunoblot of p72Plk3 and p41Plk3 in a panel of PDX cell lines grown in suspension culture for 48 h. h – j Immunoblots of p72Plk3 and p41Plk3 in HPDE cells ( h ), PATC50 cells, PANC-1 cells ( i ), and 293T cells ( j ) grown in suspension for the indicated times. k , l Immunoblot of p72Plk3, p41Plk3 expression ( k ) and flow cytometry analysis of apoptosis-inducing activity ( l ) of indicated PDAC cells that were lentivirally transduced to express the sgRNA targeting Plk3 or non-targeting control sgRNA. Cells grow on tissue culture plates (Attached) or polyHEMA-coated plates in suspension (Suspension). The p41Plk3:p72Plk3 ratios are shown at the bottom. m , n Immunoblots of p72Plk3 and p32Plk3 in PANC-1 cells grown in suspension condition. Expression of Plk3 aa 354-646 in lane 4 in ( n ) was used as a positive control. o Immunoblot of p38Plk1 expression in PANC-1 cells transfected with Myc-p68Plk1 in suspension culture for the indicated times. p Immunoblot of Plk1 cleavage in the indicated PDAC cells transfected with Plk1 shRNA. The p41Plk3:p72Plk3 ratios or p32Plk3:p72Plk3 ratios are shown at the bottom in ( f , h – j , and m ). Data are representative of two independent experiments with similar results ( a left, b – k, m – p ). n = 3 independent experiments ( a , l ). Error bars, mean ± SEM ( a right, l ), two-tailed unpaired t -test ( a right), ANOVA analysis (l ). Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: Nardilysin-regulated scission mechanism activates polo-like kinase 3 to suppress the development of pancreatic cancer

    doi: 10.1038/s41467-024-47242-3

    Figure Lengend Snippet: a Left, immunoblot of Plk3 expression under a Dox-inducible system in HPNE cells treated with Dox for the indicated times. Middle and right, apoptosis-inducing activity of HPNE/iPlk3 as evaluated using Annexin V/PI staining and flow cytometry analysis. b , c Immunoblots of cleaved PARP in HPDE cells ( b ) and PDAC cells ( c ) grown in suspension (Sus. Culture) on polyHEMA pre-coated plates at the indicated times. C.B.S., Coomassie blue–stained protein bands as a loading control. d , e Immunoblots of cleaved PARP in HPDE cells with stable shRNA-mediated knockdown of Plk3 ( d ) and stably reconstituted with Flag-Plk3 ( e ). f Immunoblot of p72Plk3 and p41Plk3 in PANC-1 cells transfected with N-terminal Flag-p72Plk3. g Immunoblot of p72Plk3 and p41Plk3 in a panel of PDX cell lines grown in suspension culture for 48 h. h – j Immunoblots of p72Plk3 and p41Plk3 in HPDE cells ( h ), PATC50 cells, PANC-1 cells ( i ), and 293T cells ( j ) grown in suspension for the indicated times. k , l Immunoblot of p72Plk3, p41Plk3 expression ( k ) and flow cytometry analysis of apoptosis-inducing activity ( l ) of indicated PDAC cells that were lentivirally transduced to express the sgRNA targeting Plk3 or non-targeting control sgRNA. Cells grow on tissue culture plates (Attached) or polyHEMA-coated plates in suspension (Suspension). The p41Plk3:p72Plk3 ratios are shown at the bottom. m , n Immunoblots of p72Plk3 and p32Plk3 in PANC-1 cells grown in suspension condition. Expression of Plk3 aa 354-646 in lane 4 in ( n ) was used as a positive control. o Immunoblot of p38Plk1 expression in PANC-1 cells transfected with Myc-p68Plk1 in suspension culture for the indicated times. p Immunoblot of Plk1 cleavage in the indicated PDAC cells transfected with Plk1 shRNA. The p41Plk3:p72Plk3 ratios or p32Plk3:p72Plk3 ratios are shown at the bottom in ( f , h – j , and m ). Data are representative of two independent experiments with similar results ( a left, b – k, m – p ). n = 3 independent experiments ( a , l ). Error bars, mean ± SEM ( a right, l ), two-tailed unpaired t -test ( a right), ANOVA analysis (l ). Source data are provided as a Source Data file.

    Article Snippet: C-Fos siRNA and scramble RNA (negative control) were purchased from Santa Cruz Biotechnology (SC-29221), and 20 nM siRNA was transfected into HPNE cells with Lipofectamine RNAiMax reagent (Life Technologies).

    Techniques: Western Blot, Expressing, Activity Assay, Staining, Flow Cytometry, Suspension, Control, shRNA, Knockdown, Stable Transfection, Transfection, Positive Control, Two Tailed Test